Composite

Part:BBa_K137109:Design

Designed by: Victoria Hsiao   Group: iGEM08_Caltech   (2008-08-22)


pabBj + b0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We wanted a functional plasmid with just pabB to test for para-aminobenzoic acid levels during overexpression.


Source

The original source of the gene is from the genomic DNA of Lactococcus lactis.

References